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Addgene inc lei lu
Lei Lu, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lei+lu/pSUPER-retro-puro-shNup35-EcoRI+(Plasmid+%2387332)/pm40411682-372-26-28
Average 93 stars, based on 3 article reviews
lei lu - by Bioz Stars, 2026-10
93/100 stars

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Plasmid Preparation:

Article Title: Estrogenic activity of E2-conjugated GLP-1 is mediated by intracellular endolysosomal acidification and estrone metabolism
Article Snippet: NES-Nluc-miniG plasmids (mGα s and mGα q ) were gifts from Kevin Pfleger (Harry Perkins Institute of Medical Research, Nedlands, WA, Australia) as originally published by Professor Nevin Lambert (Augusta University, Augusta, GA, USA) [ ]. β-arrestin 1/2-Rluc8 plasmids were a gift from Professor Terry Hébert (McGill University, Montreal, Canada). .. EGFP-CAAX was a gift from Lei Lu (Addgene plasmid # 86056). .. Lamp1-mNeonGreen was a gift from Dorus Gadella (Addgene plasmid # 98882). pEF1a-2xSV40_NLS-NLuc was a gift from Antonio Amelio (Addgene plasmid # 135953).

Article Title: Inhibition of PolyGA Dipeptide Repeat Protein Aggregation by Nucleic Acid Aptamers in C9 Amyotrophic Lateral Sclerosis-Frontotemporal Dementia Models.
Article Snippet: Hexanucleotide (GGG GCC ) repeat expansion in non-coding region of C9ORF72 is the main genetic cause of amyotrophic lateral sclerosis-frontotemporal dementia (ALS-FTD).. Gain of toxic function, via RNA or proteins, or loss of function via haploinsufficiency, are probable mechanisms of disease progression.. Expanded GGG GCC repeat codes for dipeptide repeat (DPR) proteins which form inclusions in the brain.

Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo
Article Snippet: .. The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051 ; RRID:Addgene_86051) using following primers pair: 5′- tTGTACAAGGGATCCGGAGAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/5′- aAGCGCTTCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. ..

Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo.
Article Snippet: The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051; RRID:Addgene_86051) using following primers pair: 50- tTGTACAAGGGATCCGGA GAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/50- aAGCGCT TCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. .. The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051; RRID:Addgene_86051) using following primers pair: 50- tTGTACAAGGGATCCGGA GAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/50- aAGCGCT TCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. .. Human PSC culture and cortical cell differentiation Human ESC (hESC) (H9; WiCell Cat # NIHhESC-10-0062; female donor) were grown on irradiated mouse embryonic fibroblasts (MEFs) in the ES cell medium until the start of cortical cell differentiation.

Article Title: Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell function.
Article Snippet: .. We thank Mien- Chie Hung for providing the HER2 WT plasmid (Addgene #16257), Feng Zhang for providing the lentiCRISPR v2 plasmid (Addgene #52961), Ronald D. Vale for providing the TCRα/β/CD3ε/ζ plasmid (Addgene #89347), Lei Lu for providing the CD8α–EGFP plasmid (Addgene #86051), Scott McComb for providing pSLCAR- CD19- 28z and pSLCAR- CD19- BBz plasmids (Addgene #135991 and #135992), RIKEN BRC, Yoshihide Hayashizaki and Sumio Sugano for providing the IGHG1 plasmid (IRAK174M03), George R. Stark for providing the GAS- Luc plasmid, and Toshiki Ochi for the anti- NY- ESO- 1 scFv information. ..

Article Title: Estrogenic activity of E2-conjugated GLP-1 is mediated by intracellular endolysosomal acidification and estrone metabolism.
Article Snippet: NES-Nluc-miniG plasmids (mGαs and mGαq) 437 were gifts from Kevin Pfleger (Harry Perkins Institute of Medical Research, Nedlands, WA, 438 Australia) as originally published by Professor Nevin Lambert (Augusta University, Augusta, GA, 439 USA) [36]. β-arrestin 1/2-Rluc8 plasmids were a gift from Professor Terry Hébert (McGill 440 University, Montreal, Canada). .. EGFP-CAAX was a gift from Lei Lu (Addgene plasmid # 86056). .. 441 Lamp1-mNeonGreen was a gift from Dorus Gadella (Addgene plasmid # 98882). pEF1a-442 2xSV40_NLS-NLuc was a gift from Antonio Amelio (Addgene plasmid # 135953).

Article Title: Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases
Article Snippet: Rapalog A/C heterodimerizer (Takara, Cat # 635056) was added at 5 μM final concentration. mCherry-ER-3 (mCherry-KDEL; Addgene plasmid #55041) and mEmerald- TGNP-N-10 (Addgene plasmid #54279) were gifts from Michael Davidson. mCherrySec61-β was a gift from Gia Voeltz (Zurek et al., 2011; Addgene plasmid #49155). mEmerald-Sec61β-C1 was a gift from Jennifer Lippincott-Schwartz (Nixon-Abell et al., 2016; Addgen plasmid #90992). .. The following eGFP-Rab plasmids were gifts from Marci Scidmore: eGFP-Rab6a (Rzomp et al., 2003; Addgene plasmid #49469), eGFPRab6a Q72L (Moorhead et al., 2007; Addgene plasmid #49483), eGFP-Rab6a T27N (Moorhead et al., 2007; Addgene plasmid #49484), eGFP-Rab6b (Rzomp et al., 2003; 49470), eGFP-Rab6b Q72L (Addgene plasmid #49889), eGFP-Rab6b T27N (Addgene plasmid # 49890), eGFP-Rab10 (Rzomp et al., 2003; Addgene plasmid #49472), eGFPRab10 Q68L (Huang et al., 2010; Addgene plasmid #49544), eGFP-Rab8 (Huang et al., 2010; Addgene plasmid #49543), eGFP-Rab8 T22N (Addgene plasmid #49893), eGFPRab13 (Huang et al., 2010; Addgene plasmid #49548), eGFP-Rab14 (Huang et al., 2010; Addgene plasmid #49549), and eGFP-Rab14 S25N (Addgene plasmid #49593). eGFP-Rab8 Q67L was a gift from Lei Lu (Madugula and Lu, 2016; Addgene plasmid #86076). .. GFP-Rab11 WT (Choudhury et al., 2002; Addgene plasmid #12674) and GFPRab11 DN (Choudhury et al., 2002; Addgene plasmid #12678) were gifts from Richard Pagano. eGFP-Rab13 T22N was a gift from Bernardo Mainou (Mainou and Dermody, 2012; Addgene plasmid #110501). eGFP-Rab7a was a gift from Qing Zhong (Sun et al., 2010; Addgene plasmid #28047). pmScarlet_alphaTubulin_C1 was a gift from Daphne Bindels (Bindels et al., 2017; Addgene plasmid #85045). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Ran et al., 2013; Addgne plasmid #62988).

Article Title: Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases
Article Snippet: To visualize mitochondria, 0.2 μl of 1mM Mito-tracker Deep Red was added 60 mins prior to imaging. mCherry-ER-3 (mCherry-KDEL; Addgene plasmid #55041) and mEmerald-TGNP-N-10 (Addgene plasmid #54279) were gifts from Michael Davidson. mCherry-Sec61-β was a gift from Gia Voeltz ( Zurek et al ., 2011 ; Addgene plasmid #49155). mEmerald-Sec61β-C1 was a gift from Jennifer Lippincott-Schwartz ( Nixon-Abell et al ., 2016 ; Addgene plasmid #90992). .. The following eGFP-Rab plasmids were gifts from Marci Scidmore: eGFP-Rab6a ( Rzomp et al ., 2003 ; Addgene plasmid #49469), eGFP-Rab6a Q72L ( Moorhead et al ., 2007 ; Addgene plasmid #49483), eGFP-Rab6a T27N ( Moorhead et al ., 2007 ; Addgene plasmid #49484), eGFP-Rab6b (Rzomp et al., 2003( Rzomp et al ., 2003 ; Addgene plasmid #49470), eGFP-Rab6b Q72L (Addgene plasmid #49889), eGFP-Rab6b T27N (Addgene plasmid # 49890), eGFP-Rab10 ( Rzomp et al ., 2003 ; Addgene plasmid #49472), eGFP-Rab10 Q68L ( Huang et al ., 2010 ; Addgene plasmid #49544), eGFP-Rab8 ( Huang et al ., 2010 ; Addgene plasmid #49543), eGFP-Rab8 T22N (Addgene plasmid #49893), eGFP-Rab13 (( Huang et al ., 2010 ; Addgene plasmid #49548), eGFP-Rab14 ( Huang et al ., 2010 ; Addgene plasmid #49549), and eGFP-Rab14 S25N (Addgene plasmid #49593). eGFP-Rab8 Q67L was a gift from Lei Lu ( ; Addgene plasmid #86076). .. GFP-Rab11 WT ( Choudhury et al ., 2002 ; Addgene plasmid #12674) and GFP-Rab11 DN ( Choudhury et al ., 2002 ; Addgene plasmid #12674) were gifts from Richard Pagano. eGFP-Rab13 T22N was a gift from Bernardo Mainou ( ; Addgene plasmid #110501). eGFP-Rab7a was a gift from Qing Zhong ( Sun et al ., 2010 ; Addgene plasmid #28047). pmScarlet_alphaTubulin_C1 was a gift from Daphne Bindels ( Bindels et al ., 2017 ; Addgene plasmid #85045). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang ( Ran et al ., 2013 ; Addgne plasmid #62988).

Amplification:

Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo
Article Snippet: .. The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051 ; RRID:Addgene_86051) using following primers pair: 5′- tTGTACAAGGGATCCGGAGAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/5′- aAGCGCTTCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. ..

Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo.
Article Snippet: The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051; RRID:Addgene_86051) using following primers pair: 50- tTGTACAAGGGATCCGGA GAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/50- aAGCGCT TCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. .. The T2A-tCD8 fragment was amplified from CD8a-EGFP was a gift from Lei Lu (Addgene plasmid # 86051; http://n2t.net/addgene:86051; RRID:Addgene_86051) using following primers pair: 50- tTGTACAAGGGATCCGGA GAGGGGAGAGGATCACTGCTGACTTGCGGGGATGTGGAAGAGAACCCAGGGCCCATGGCCTTACCAGTGACC-3’/50- aAGCGCT TCAGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG-3’. .. Human PSC culture and cortical cell differentiation Human ESC (hESC) (H9; WiCell Cat # NIHhESC-10-0062; female donor) were grown on irradiated mouse embryonic fibroblasts (MEFs) in the ES cell medium until the start of cortical cell differentiation.



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Addgene inc lei lu
Lei Lu, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lei+lu/pSUPER-retro-puro-shNup35-EcoRI+(Plasmid+%2387332)/pm40411682-372-26-28
Average 93 stars, based on 1 article reviews
lei lu - by Bioz Stars, 2026-10
93/100 stars
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